Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available.
Do Jurkat cells have TCR?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.
Is Jurkat a CD4?
Jurkat is a human CD4+-T-cell line that expresses CD3 along with a number of other T-cell surface receptors which are typical of freshly isolated CD4+ human peripheral blood T cells.
Where do Jurkat cells come from?
The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14].
What is a Jurkat cell line?
From Wikipedia, the free encyclopedia. Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.
Are Jurkat cells suspension or adherent?
Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts.
Are Jurkat cells CD3 positive?
I noticed that although Jurkat cells are T cells, their positive signals for CD3 antibodies were not so high. When stained, the signal does shift to the right, but does not shift so dramatically that when I try to gate, some (30-40%) of the population still remain in the region where unstained cells sit.
What do Jurkat cells do?
Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.
Do Jurkat cells express CD3?
Jurkat clones express different levels of CD3 antigen: low on 217.6 clone (A), high on 217.9 (B) and ranging from low to high levels on clone 217.7 (C).
Do Jurkat cells express CD8?
All Answers (1) The Jurkat T cell leukemic line E6–1 expresses high levels of mature TCR and low levels of CD4, but no CD8 or MHC class II.
What is the ATCC number for Jurkat e6-1?
Jurkat (ATCC number TIB-152) cell culture and formaldehyde cross- linking Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts.
What is the Jurkat T cell line used for?
The Jurkat T cell line and the many subclones of Jurkat T cells have been instrumental in studying molecular and cellular events in the HIV life cycle.
What is the karyotype of Jurkat e6-1?
Jurkat (ATCC number TIB-152) cell culture and formaldehyde cross- linking Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts. The karyotype is pseudodiploid human male cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%.
Is this a clone of the Jurkat-FHCRC cell line?
This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line. Male This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%.
