Warthin-Starry (WS) staining is an ancillary stain used in the detection of Helicobacter sp., spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of H. pylori diagnosis in gastric biopsies in comparison with anti-H.
What stain is used for Helicobacter pylori?
The diagnosis of H. pylori could be performed in hematoxylin and eosin (H&E) staining, however the specificity can be improved by special stains such as modified Giemsa, Warthin-Starry silver, Genta, and immunohistochemical (IHC) stains.
What is immunohistochemical stain for H. pylori?
pylori- associated gastritis, and it is standard practice to routinely perform histochemical or immunohistochemical stains on endoscopic gastric biopsies. Several studies have shown that immunohistochemical staining with specific H. pylori antibodies shows the highest sensitivity and specificity in detecting H. pylori.
How is H. pylori detected in biopsy?
Polymerase chain reaction. Since the application of polymerase chain reaction (PCR) to detect H. pylori infection, PCR has been used extensively for the diagnosis of H. pylori from gastric biopsy specimens, saliva, stool, gastric juice and variable specimens.
What is warthin tumor?
Warthin tumor is a benign tumor of the salivary gland. The first symptom is usually a painless, slow-growing bump in front of the ear, on the bottom of the mouth, or under the chin. Warthin tumors may increase in size over time, but few become cancerous.
What is Steiner silver stain?
The modified Steiner stain is a non-specific silver stain for identifying bacteria in formalin-fixed, paraffin-embedded tissues. The principle behind its use is that bacteria are first sensitized using uranyl nitrate solution, making them able to precipitate silver from a silver nitrate solution.
What is the most accurate test for H. pylori?
Evidence-Based Answer. When compared with serology or stool antigen tests, the urea breath test has the highest diagnostic accuracy to identify H. pylori infection in patients without a history of gastrectomy or recent use of antibiotics or proton pump inhibitors.
How does CLO test work?
The CLO testing is performed during an endoscopy. The scope used for the endoscopy will search your stomach for any type of visible damage, and if any is found, the CLO test biopsy test will be conducted. A sample is taken and then put in a medium that contains urea.
How do you get H. pylori?
H. pylori bacteria may be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori may also be spread through contaminated food or water.
