Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.
What is pGL3?
Description. The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors.
What is pGL3 basic vector?
What is a plasmid Addgene?
Plasmids 101. Plasmids are circular pieces of DNA found in bacterial cells that replicate independently from the host’s chromosome. They’re powerful and ubiquitous tools that touch nearly all areas of biological research.
Is a reporter a plasmid?
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. For bacteria or prokaryotic cells in culture, this is usually in the form of a circular DNA molecule called a plasmid.
What is pcdna3?
This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family.
What is Renilla luciferase?
Renilla luciferase is an enzyme that catalyzes the chemical reaction which is responsible for the green bioluminescence of Renilla reniformis, a soft coral also known as sea pansy and a member of the class Anthozoa [1].
How do you get plasmids from Addgene?
Addgene provides you with two spots of DNA for each plasmid. It can be stored at 4°C. To recover your plasmid: To recover the plasmid, use a clean razor blade to cut out one of the circles containing your DNA.
How do you order plasmids from Addgene?
Ordering Directly through Addgene’s Website
- Order directly by logging in and proceeding through checkout at addgene.org.
- Addgene assists with the MTA paperwork process.
- Plasmids are shipped directly to your lab.
- Payment is processed through Addgene’s US headquarters.
What is a reporter mouse?
A reporter Knockin mouse defines an animal model in which fluorescent, bioluminescent proteins or biochemical tags are inserted into the genome. The reporter can replace a gene, be fused to a protein or inserted into the 3′ UTR.
