The standard method for inhibiting microRNA (miRNA) function is by steric blocking, using an oligonucleotide that is perfectly complementary to the mature miRNA target. These inhibitors form a duplex with the miRNA guide strand and prevent the miRNA from binding to its intended target.
What is a MicroRNA inhibitor?
miRNA inhibitors are natural or artificial RNA transcripts that sequestrate miRNAs and decrease or even eliminate their depressing effects. Competing endogenous RNAs (ceRNAs) can be viewed as natural and endogenous miRNA inhibitors.
How do you test for MicroRNA?
Traditional methods for detection of miRNAs include Northern blotting, quantitative reverse transcription polymerase chain reaction (qRT-PCR), next-generation sequencing, and microarray-based hybridization (5, 8, 9).
How does miRNA inhibit translation?
We provide evidence that a miRNA can affect translation initiation by inhibiting the roles of the mRNA cap structure and poly(A) tail, in the absence of accelerated mRNA decay.
What is anti miR?
Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation.
How do Antagomirs work?
Antagomirs are microRNA inhibitors that inhibit miRNAs but, because of the promiscuity of microRNAs, antagomirs could affect the regulation of many different mRNA molecules. If a Blockmir binds to a non-intended RNA, it will only cause an effect if it prevents binding of a microRNA or another cellular factor.
What regulates miRNA?
miRNA biogenesis is regulated at multiple levels, including at the level of miRNA transcription; its processing by Drosha and Dicer in the nucleus and cytoplasm, respectively; its modification by RNA editing, RNA methylation, uridylation and adenylation; Argonaute loading; and RNA decay.
